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Stimulated Raman spectroscopy has become a powerful tool to study the spatiodynamics of molecular bonds with high sensitivity resolution and speed However the sensitivity and speed of state-of-the-art stimulated Raman scattering spectroscopy are currently limited by the shot-noise of the light beam probing the Raman process Here we demonstrate in a proof-of-principle experiment an
1 ! 1! Rapid intraoperative histology of unprocessed surgical specimens 2! via fiber-laser-based stimulated Raman scattering microscopy 3! 4! Daniel A Orringer 1* Balaji Pandian 1 Yashar S Niknafs 1 Todd C Hollon 1 Julianne Boyle 1 5! Spencer Lewis 1 Mia Garrard 1 Shawn L Hervey-Jumper 1 Hugh J L Garton 1 Cormac O
Rapid histology of brain tissues with sufficient diagnostic information has the great potential to aid neurosurgeons during operations Stimulated Raman Scattering (SRS) microscopy is an emerging label-free imaging technique with the intrinsic chemical resolutions
Here we present the first applications of stimulated Raman scattering (SRS) microscopy a nonlinear optical Raman technique to samples of mineralogical interest Combined with second harmonic generation microscopy SRS offers a multimodal tool for rapid imaging of mineral samples with chemical specificity structural sensitivity and excellent three‐dimensional resolution
Rapid 3D Chemical Profiling of Individual Atmospheric Aerosols with Stimulated Raman Scattering Microscopy Jianpeng Ao Yiqing Feng Simin Wu Tao Wang Jiwei Ling Liwu Zhang * and Minbiao Ji* J Ao S Wu J Ling Prof M Ji State Key Laboratory of
Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line RUOYU HE 1 ZHIPING LIU 1 YONGKUI XU 1 WEI HUANG 2 3 HONG MA 2 3 AND MINBIAO JI1 3 * 1State Key Laboratory of Surface Physics and Department of Physics Fudan University Shanghai 200433 China
Spectroscopic stimulated Raman scattering (SRS) microscopy is a label-free technique that generates chemical maps of live cells or tissues A handheld SRS imaging system using an optical fiber for laser delivery will further enable in situ and in vivo compositional analysis for applications such as medical diagnosis and surgical guidance In fiber-delivered SRS the interaction of two
Fig 6 Label free microscopy of squamous cell carcinoma cells by stimulated Raman scattering microscopy (A) Stimulated Raman scattering image of fixed SCC cells (i) acquired at 2953 cm −1 (CH 3) (ii) acquired at 2850 cm −1 (CH 2 lipid) (iii) acquired at −1
Stimulated Raman scattering (SRS) microscopy based on the coherent interaction of 2 different laser beams with vibrational levels in the molecules of the sample would enable much faster detection and identification of microplastics
Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line RUOYU HE 1 ZHIPING LIU 1 YONGKUI XU 1 WEI HUANG 2 3 HONG MA 2 3 AND MINBIAO JI1 3 * 1State Key Laboratory of Surface Physics and Department of Physics Fudan University Shanghai 200433 China
Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens We have developed a system based on spectral focusing SRS technology with the integration of a rapid scanning optical delay line which allows continuous tuning of SRS spectra by scanning a
Here a phenotypic platform that rapidly produces AST results by femtosecond stimulated Raman scattering imaging of deuterium oxide (D 2 O) metabolism is reported Metabolic incorporation of D 2 O into biomass in a single bacterium and the metabolic response to antibiotics are probed in as short as 10 min after culture in 70% D 2 O medium the fastest among current technologies
Here a phenotypic platform that rapidly produces AST results by femtosecond stimulated Raman scattering imaging of deuterium oxide (D 2 O) metabolism is reported Metabolic incorporation of D 2 O into biomass in a single bacterium and the metabolic response to antibiotics are probed in as short as 10 min after culture in 70% D 2 O medium the fastest among current technologies
Stimulated Raman scattering (SRS) microscopy In SRS the intensity of the energy transfer from the pump wavelength to the Stokes laser wavelength is measured as a signal There are two ways to measure SRS signals one is to measure the increase of power in Stokes laser which is called stimulated Raman gain (SRG)
The scheme combines the concept from stimulated depletion microscopy with femtosecond stimulated Raman scattering The suppression of the Raman signal in a three-beam setup with only two involved wavelength-components was accomplished by the saturation of the Raman scattering
Fig 6 Label free microscopy of squamous cell carcinoma cells by stimulated Raman scattering microscopy (A) Stimulated Raman scattering image of fixed SCC cells (i) acquired at 2953 cm −1 (CH 3) (ii) acquired at 2850 cm −1 (CH 2 −1 (C
Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens We have developed a system based on spectral focusing SRS technology with the integration of a rapid scanning optical delay line which allows continuous tuning of SRS spectra by scanning a
Here we present the first applications of stimulated Raman scattering (SRS) microscopy a nonlinear optical Raman technique to samples of mineralogical interest Combined with second harmonic generation microscopy SRS offers a multimodal tool for rapid imaging of mineral samples with chemical specificity structural sensitivity and excellent three‐dimensional resolution
Stimulated Raman scattering (SRS) microscopy a label-free optical process has been shown to rapidly detect brain-tumour infiltration in fresh unprocessed human tissues Here we demonstrate the first application of SRS microscopy in the operating room by using a portable fibre-laser-based microscope and unprocessed specimens from 101 neurosurgical patients
Rapid 3D Chemical Profiling of Individual Atmospheric Aerosols with Stimulated Raman Scattering Microscopy Jianpeng Ao Yiqing Feng Simin Wu Tao Wang Jiwei Ling Liwu Zhang * and Minbiao Ji* J Ao S Wu J Ling Prof M Ji State Key Laboratory of
2019/4/13precise and rapid intraoperative histology of laryngeal tissue is crucial for providing optimal surgical outcomes We hypothesized that deep-learning based stimulated Raman scattering (SRS) microscopy could provide automated and accurate diagnosis of
(2019) Stimulated Raman Scattering Microscopy for Brain Imaging: Basic Principle Measurements and Applications In: Kao FJ Keiser G Gogoi A (eds) Advanced Optical Methods for Brain Imaging Progress in Optical Science and Photonics vol 5
Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens We have developed a system based on spectral focusing SRS technology with the integration of a rapid scanning optical delay line which allows continuous tuning of SRS spectra by scanning a
1 Rapid histology of laryngeal squamous cell carcinoma with deep-learning based stimulated Raman scattering microscopy Lili Zhang1 2# Yongzheng Wu1# Bin Zheng3#* Lizhong Su3 Yuan Chen4 Shuang Ma4 Qinqin Hu4 Xiang Zou5 Lie Yao5 Yinlong Yang6 Liang Chen5 Ying Mao5
Stimulated Raman scattering microscopy has emerged as a new frontier in light microscopy with exciting developments Recently heavy water and deuterated glucose were applied for metabolic imaging in vivo which allowed visualization of proteins lipids DNA and glycogen turnover in animals
Stimulated Raman spectroscopy also referred to as stimulated raman scattering (SRS) is a form of spectroscopy employed in physics chemistry biology and other fields The basic mechanism resembles that of spontaneous Raman spectroscopy: a pump photon of the angular frequency which is absorbed by a molecule has some small probability of inducing some vibrational (or rotational) transition
2020/1/11 Theranostics 2020 Jan 1 10(1):312-322 doi: 10 7150/thno 38551 eCollection 2020 Spatial light-modulated stimulated Raman scattering (SLM-SRS) microscopy for rapid multiplexed vibrational imaging Bae K(1) Zheng W(1) Huang Z(1) Author information: (1)Optical Bioimaging Laboratory Department of Biomedical Engineering Faculty of Engineering National University of Singapore
Fig 6 Label free microscopy of squamous cell carcinoma cells by stimulated Raman scattering microscopy (A) Stimulated Raman scattering image of fixed SCC cells (i) acquired at 2953 cm −1 (CH 3) (ii) acquired at 2850 cm −1 (CH 2 lipid) (iii) acquired at −1
Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line RUOYU HE 1 ZHIPING LIU 1 YONGKUI XU 1 WEI HUANG 2 3 HONG MA 2 3 AND MINBIAO JI1 3 * 1State Key Laboratory of Surface Physics and Department of Physics Fudan University Shanghai 200433 China
2017/6/24Stimulated Raman scattering microscopy for rapid brain tumor histology Yang* Lingchao Chen† and Minbiao Ji* ‡ *State Key Laboratory of Surface Physics and Department of Physics Key Laboratory of Micro and Nano Photonic Structures (Ministry of
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